Molecular characterization and phylogenetic analysis of bovine viral diarrhea virus in dairy herds of Fars province, Iran.

ABSTRACT: Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. The aim of present study was to determine the molecular characterization and phylogenetic analysis of BVDV infection in dairy herds of Fars province, Iran. For initial screening, a total of 400 blood samples were collected from 12 industrial dairy herds with previous history of diarrhea, abortion or birth of weak calves and analyzed using reverse transcription-polymerase chain reaction (RT-PCR) on buffy coat. In the next step, blood samples and also ear notch biopsies were collected from 100 cattle of infected farms three weeks later which were subsequently tested by antigen capture ELISA (ACE), RT-PCR and immunohistochemistry (IHC). The results of nested RT-PCR were successful in 16 out of

SUBMITTER: Khodakaram-Tafti A 

PROVIDER: S-EPMC5090137 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature