Towards the Analysis of High Molecular Weight Proteins and Protein complexes using TIMS-MS.

ABSTRACT: In the present work, we demonstrate the potential and versatility of TIMS for the analysis of proteins, DNA-protein complexes and protein-protein complexes in their native and denatured states. In addition, we show that accurate CCS measurement are possible and in good agreement with previously reported CCS values using other IMS analyzers (<5% difference). The main challenges for the analysis of high mass proteins and protein complexes in the mobility and m/z domain are described. That is, the analysis of high molecular weight systems in their native state may require the use of higher electric fields or a compromise in the TIMS mobility resolution by reducing the bath gas velocity in order to effectively trap at lower electric fields. This is the first report of CCS measurements o

SUBMITTER: Benigni P 

PROVIDER: S-EPMC5091298 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature