Novel nuclei isolation buffer for flow cytometric genome size estimation of Zingiberaceae: a comparison with common isolation buffers.

ABSTRACT:

Background and aims

Cytological parameters such as chromosome numbers and genome sizes of plants are used routinely for studying evolutionary aspects of polyploid plants. Members of Zingiberaceae show a wide range of inter- and intrageneric variation in their reproductive habits and ploidy levels. Conventional cytological study in this group of plants is severely hampered by the presence of diverse secondary metabolites, which also affect their genome size estimation using flow cytometry. None of the several nuclei isolation buffers used in flow cytometry could be used very successfully for members of Zingiberaceae to isolate good quality nuclei from both shoot and root tissues.

Methods

The competency of eight nuclei isolation buffers was compared with a newly formulated buf

SUBMITTER: Sadhu A 

PROVIDER: S-EPMC5091729 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature