Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production.

ABSTRACT: Yarrowia lipolytica is a non-pathogenic, dimorphic and strictly aerobic yeast species. Owing to its distinctive physiological features and metabolic characteristics, this unconventional yeast is not only a good model for the study of the fundamental nature of fungal differentiation but is also a promising microbial platform for biochemical production and various biotechnological applications, which require extensive genetic manipulations. However, genetic manipulations of Y. lipolytica have been limited due to the lack of an efficient and stable genetic transformation system as well as very high rates of non-homologous recombination that can be mainly attributed to the KU70 gene. Here, we report an easy and rapid protocol for the efficient genetic transformation and for gene deletion in Y.

SUBMITTER: Yu AQ 

PROVIDER: S-EPMC5092062 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature