Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer.

ABSTRACT: Recent improvements in Förster resonance energy transfer (FRET) sensors have enabled their use to detect various small molecules including ions and amino acids. However, the innate weak signal intensity of FRET sensors is a major challenge that prevents their application in various fields and makes the use of expensive, high-end fluorometers necessary. Previously, we built a cost-effective, high-performance FRET analyzer that can specifically measure the ratio of two emission wavelength bands (530 and 480 nm) to achieve high detection sensitivity. More recently, it was discovered that FRET sensors with bacterial periplasmic binding proteins detect ligands with maximum sensitivity in the critical temperature range of 50 - 55 °C. This report describes a protocol for assessing sugar content i

SUBMITTER: Kim H 

PROVIDER: S-EPMC5092083 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature