Insights into Protein Structure and Dynamics by Ultraviolet and Visible Resonance Raman Spectroscopy.

ABSTRACT: Raman spectroscopy is a form of vibrational spectroscopy based on inelastic scattering of light. In resonance Raman spectroscopy, the wavelength of the incident light falls within an absorption band of a chromophore, and this overlap of excitation and absorption energy greatly enhances the Raman scattering efficiency of the absorbing species. The ability to probe vibrational spectra of select chromophores within a complex mixture of molecules makes resonance Raman spectroscopy an excellent tool for studies of biomolecules. In this Current Topic, we discuss the type of molecular insights obtained from steady-state and time-resolved resonance Raman studies of a prototypical photoactive protein, rhodopsin. We also review recent efforts in ultraviolet resonance Raman investigations of soluble

SUBMITTER: Lopez-Pena I 

PROVIDER: S-EPMC5092233 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature