Inhibition of SOAT1 Suppresses Glioblastoma Growth via Blocking SREBP-1-Mediated Lipogenesis.

ABSTRACT:

Purpose

Elevated lipogenesis regulated by sterol regulatory element-binding protein-1 (SREBP-1), a transcription factor playing a central role in lipid metabolism, is a novel characteristic of glioblastoma (GBM). The aim of this study was to identify effective approaches to suppress GBM growth by inhibition of SREBP-1. As SREBP activation is negatively regulated by endoplasmic reticulum (ER) cholesterol, we sought to determine whether suppression of sterol O-acyltransferase (SOAT), a key enzyme converting ER cholesterol to cholesterol esters (CE) to store in lipid droplets (LDs), effectively suppressed SREBP-1 and blocked GBM growth.

Experimental design

The presence of LDs in glioma patient tumor tissues was analyzed using immunofluorescence, immunohistochemistry, and electr

SUBMITTER: Geng F 

PROVIDER: S-EPMC5093025 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature