Comparison of Collection Methods for Fecal Samples for Discovery Metabolomics in Epidemiologic Studies.

ABSTRACT:

Background

The gut metabolome may be associated with the incidence and progression of numerous diseases. The composition of the gut metabolome can be captured by measuring metabolite levels in the feces. However, there are little data describing the effect of fecal sample collection methods on metabolomic measures.

Methods

We collected fecal samples from 18 volunteers using four methods: no solution, 95% ethanol, fecal occult blood test (FOBT) cards, and fecal immunochemical test (FIT). One set of samples was frozen after collection (day 0), and for 95% ethanol, FOBT, and FIT, a second set was frozen after 96 hours at room temperature. We evaluated (i) technical reproducibility within sample replicates, (ii) stability after 96 hours at room temperature for 95% ethanol, FOBT,

SUBMITTER: Loftfield E 

PROVIDER: S-EPMC5093035 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature