Improving virus production through quasispecies genomic selection and molecular breeding.

ABSTRACT: Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in

SUBMITTER: Perez-Rodriguez FJ 

PROVIDER: S-EPMC5093897 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature