Controlled dehydration improves the diffraction quality of two RNA crystals.

ABSTRACT:

Background

Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals.

Results

We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders.

Conclusion

The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structu

SUBMITTER: Park H 

PROVIDER: S-EPMC5093936 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature