Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus.

ABSTRACT:

Background

Lumpy skin disease virus (LSDV) is a Capripoxvirus infecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed.

Results

The LSDV RPA assay was performed at 42 °C a

SUBMITTER: Shalaby MA 

PROVIDER: S-EPMC5094145 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature