Resolution of ab initio shapes determined from small-angle scattering.

ABSTRACT: Spatial resolution is an important characteristic of structural models, and the authors of structures determined by X-ray crystallography or electron cryo-microscopy always provide the resolution upon publication and deposition. Small-angle scattering of X-rays or neutrons (SAS) has recently become a mainstream structural method providing the overall three-dimensional structures of proteins, nucleic acids and complexes in solution. However, no quantitative resolution measure is available for SAS-derived models, which significantly hampers their validation and further use. Here, a method is derived for resolution assessment for ab initio shape reconstruction from scattering data. The inherent variability of the ab initio shapes is utilized and it is demonstrated how their aver

SUBMITTER: Tuukkanen AT 

PROVIDER: S-EPMC5094446 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature