The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation.

ABSTRACT: Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved in a cell response to the combined stress factors resulted from protoplast generation. Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term stress on the chloroplast proteome. Using label-free comparative quantitative proteomic analysis (SWATH-MS), we quantified 479 chloroplast proteins, 219 of which showed a more than 1.4-fold change in abundance in protoplasts. We additionally quantified 1451 chloroplast proteins usi

SUBMITTER: Fesenko I 

PROVIDER: S-EPMC5095126 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature