Role of intracellular poroelasticity on freezing-induced deformation of cells in engineered tissues.

ABSTRACT: Freezing of biomaterials is important in a wide variety of biomedical applications, including cryopreservation and cryosurgeries. For the success of these applications to various biomaterials, biophysical mechanisms, which determine freezing-induced changes in cells and tissues, need to be well understood. Specifically, the significance of the intracellular mechanics during freezing is not well understood. Thus, we hypothesize that cells interact during freezing with the surroundings such as suspension media and the extracellular matrix (ECM) via two distinct but related mechanisms-water transport and cytoskeletal mechanics. The underlying rationale is that the cytoplasm of the cells has poroelastic nature, which can regulate both cellular water transport and cytoskeletal mechanics. A poro

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC5095213 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature