Label-free in situ imaging of oil body dynamics and chemistry in germination.

ABSTRACT: Plant oleosomes are uniquely emulsified lipid reservoirs that serve as the primary energy source during seed germination. These oil bodies undergo significant changes regarding their size, composition and structure during normal seedling development; however, a detailed characterization of these oil body dynamics, which critically affect oil body extractability and nutritional value, has remained challenging because of a limited ability to monitor oil body location and composition during germination in situ Here, we demonstrate via in situ, label-free imaging that oil bodies are highly dynamic intracellular organelles that are morphologically and biochemically remodelled extensively during germination. Label-free, coherent Raman microscopy (CRM) combined with bulk biochemical measurements

SUBMITTER: Waschatko G 

PROVIDER: S-EPMC5095225 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature