18F-FNDP for PET Imaging of Soluble Epoxide Hydrolase.

ABSTRACT: Soluble epoxide hydrolase (sEH) is a bifunctional enzyme located within cytosol and peroxisomes that converts epoxides to the corresponding diols and hydrolyzes phosphate monoesters. It serves to inactivate epoxyeicosatrienoic acids (EETs), which are generated in the brain to couple neuronal activity and cerebral blood flow in normal and pathologic states. Altered regulation of sEH was observed previously in various neuropathologic disorders including vascular dementia and stroke. Inhibitors of sEH are pursued as agents to mitigate neuronal damage after stroke. We developed N-(3,3-diphenylpropyl)-6-¹⁸F-fluoronicotinamide (¹⁸F-FNDP), which proved highly specific for imaging of sEH in the mouse and nonhuman primate brain with PET.

Methods

¹⁸F-FNDP was

SUBMITTER: Horti AG 

PROVIDER: S-EPMC5095511 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature