In situ embryo rescue for generation of wide intra- and interspecific hybrids of Panicum virgatum L.

ABSTRACT: Wide crosses have been used for decades as a method for transferring novel genetic material and traits in plant breeding. Historically, many products of wide crosses require tedious and inefficient surgical embryo rescue prior to embryo abortion to recover single plantlets. We have utilized transgenic switchgrass (Panicum virgatum L. cv Alamo) as a pollen donor in conjunction with antibiotic or herbicide selection for recovery of intra-and interspecific F₁ crosses by using developing ovules from the female parent and selecting for embryogenic cultures derived from the in situ immature embryo. Using this approach, several intravarietial crosses were generated between transgenic Alamo and the switchgrass varieties Kanlow, Blackwell and Cave-in-Rock as well as an interspecific cros

SUBMITTER: Kausch AP 

PROVIDER: S-EPMC5095774 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature