A standardized production pipeline for high profile targets from Mycobacterium tuberculosis.

ABSTRACT:

Purpose

Tuberculosis is still a major threat to global health. New tools and strategies to produce disease-related proteins are quintessential for the development of novel vaccines and diagnostic markers.

Experimental design

To obtain recombinant proteins from Mycobacterium tuberculosis (Mtb) for use in clinical applications, a standardized procedure was developed that includes subcloning, protein expression in Mycobacterium smegmatis and protein purification using chromatography. The potential for the different protein targets to serve as diagnostic markers for tuberculosis was established using multiplex immunoassays.

Results

Twelve soluble proteins from Mtb, including one protein complex, were purified to near-homogeneity following recombinant expression in M. smeg

SUBMITTER: Milewski MC 

PROVIDER: S-EPMC5095800 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature