Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes.

ABSTRACT:

Background

Grain development in maize is an essential process in the plant's life cycle and is vital for use of the plant as a crop for animals and humans. However, little is known regarding the protein regulatory networks that control grain development. Here, isobaric tag for relative and absolute quantification (iTRAQ) technology was used to analyze temporal changes in protein expression during maize grain development.

Results

Maize grain proteins and changes in protein expression at eight developmental stages from 3 to 50 d after pollination (DAP) were performed using iTRAQ-based proteomics. Overall, 4751 proteins were identified; 2639 of these were quantified and 1235 showed at least 1.5-fold changes in expression levels at different developmental stages and were identif

SUBMITTER: Yu T 

PROVIDER: S-EPMC5095984 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature