Moraxella catarrhalis induces CEACAM3-Syk-CARD9-dependent activation of human granulocytes.

ABSTRACT: The human restricted pathogen Moraxella catarrhalis is an important causal agent for exacerbations in chronic obstructive lung disease in adults. In such patients, increased numbers of granulocytes are present in the airways, which correlate with bacteria-induced exacerbations and severity of the disease. Our study investigated whether the interaction of M. catarrhalis with the human granulocyte-specific carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-3 is linked to NF-κB activation, resulting in chemokine production. Granulocytes from healthy donors and NB4 cells were infected with M. catarrhalis in the presence of different inhibitors, blocking antibodies and siRNA. The supernatants were analysed by enzyme-linked immunosorbent assay for chemokines. NF-κB activation was d

SUBMITTER: Heinrich A 

PROVIDER: S-EPMC5096018 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature