Tackling aspecific side reactions during histone propionylation: The promise of reversing overpropionylation.

ABSTRACT: Histone proteins are essential elements for DNA packaging. Moreover, the PTMs that are extremely abundant on these proteins, contribute in modeling chromatin structure and recruiting enzymes involved in gene regulation, DNA repair and chromosome condensation. This fundamental aspect, together with the epigenetic inheritance of histone PTMs, underlines the importance of having biochemical techniques for their characterization. Over the past two decades, significant improvements in mass accuracy and resolution of mass spectrometers have made LC-coupled MS the strategy of choice for accurate identification and quantification of protein PTMs. Nevertheless, in previous work we disclosed the limitations and biases of the most widely adopted sample preparation protocols for histone propionylation

SUBMITTER: Meert P 

PROVIDER: S-EPMC5096241 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature