Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area.

ABSTRACT:

Background

Plasmodium falciparum resistance to artemisinin emerged in the Greater Mekong Sub-region has been associated with mutations in the propeller domain of the kelch gene Pfk13.

Methods

Here the polymorphisms in Pvk12 gene, the orthologue of Pfk13 in Plasmodium vivax, were determined by PCR and sequencing in 262 clinical isolates collected in recent years (2012-2015) from the China-Myanmar border area.

Results

Sequencing of full-length Pvk12 genes from these isolates identified three synonymous mutations (N172N, S360S, S697S) and one non-synonymous mutation M124I, all of which were at very low prevalence (2.0-3.1%). Moreover, these mutations were non-overlapping between the two study sites on both sides of the border. Molecular evolutionary analysis detected sig

SUBMITTER: Deng S 

PROVIDER: S-EPMC5096284 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature