Use of Dried Blood Spots to Elucidate Full-Length Transmitted/Founder HIV-1 Genomes.

ABSTRACT:

Background

Identification of HIV-1 genomes responsible for establishing clinical infection in newly infected individuals is fundamental to prevention and pathogenesis research. Processing, storage, and transportation of the clinical samples required to perform these virologic assays in resource-limited settings requires challenging venipuncture and cold chain logistics. Here, we validate the use of dried-blood spots (DBS) as a simple and convenient alternative to collecting and storing frozen plasma.

Methods

We performed parallel nucleic acid extraction, single genome amplification (SGA), next generation sequencing (NGS), and phylogenetic analyses on plasma and DBS.

Results

We demonstrated the capacity to extract viral RNA from DBS and perform SGA to infer the complet

SUBMITTER: Salazar-Gonzalez JF 

PROVIDER: S-EPMC5096837 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature