Human parainfluenza virus-3 can be targeted by rapidly ex vivo expanded T lymphocytes.

ABSTRACT:

Background aims

Human parainfluenza virus-3 (HPIV) is a common cause of respiratory infection in immunocompromised patients and currently has no effective therapies. Virus-specific T-cell therapy has been successful for the treatment or prevention of viral infections in immunocompromised patients but requires determination of T-cell antigens on targeted viruses.

Methods

HPIV3-specific T cells were expanded from peripheral blood of healthy donors using a rapid generation protocol targeting four HPIV3 proteins. Immunophenotyping was performed by flow cytometry. Viral specificity was determined by interferon (IFN)-γ ELISpot, intracellular cytokine staining and cytokine measurements from culture supernatants by Luminex assay. Cytotoxic activity was tested by ⁵¹Cr rele

SUBMITTER: McLaughlin LP 

PROVIDER: S-EPMC5096976 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature