P53-dependent up-regulation of CDKN1A and down-regulation of CCNE2 in response to beryllium.

ABSTRACT:

Objectives

Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing transcription factor, in mediating beryllium-induced cytostasis.

Materials and methods

Human A172 glioma cells express wild-type TP53 gene. Activity of p53 was experimentally manipulated using siRNA and related approaches. Key elements of the beryllium-response were compared in normal and p53-knockdown A172 cells using RT-PCR and Western blotting.

Results

In A172 cells, 10 μm BeSO₄ caused 300% increase in CDKN1A (cyclin-dependent kinase inhib

SUBMITTER: Gorjala P 

PROVIDER: S-EPMC5096984 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature