Identification of microRNA profile specific to cancer stem-like cells directly isolated from human larynx cancer specimens.

ABSTRACT:

Background

Emerging evidences proposed that microRNAs are associated with regulation of distinct physio-pathological processes including development of normal stem cells and carcinogenesis. In this study we aimed to investigate microRNA profile of cancer stem-like cells (CSLCs) isolated form freshly resected larynx cancer (LCa) tissue samples.

Methods

CD133 positive (CD133⁺) stem-like cells were isolated from freshly resected LCa tumor specimens. MicroRNA profile of 12 pair of CD133⁺ and CD133^- cells was determined using microRNA microarray and differential expressions of selvected microRNAs were validated by quantitative real time PCR (qRT-PCR).

Results

MicroRNA profiling of CD133⁺ and CD133^- LCa samples with mic

SUBMITTER: Karatas OF 

PROVIDER: S-EPMC5097853 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature