Insight into the Exoproteome of the Tissue-Derived Trypomastigote form of Trypanosoma cruzi.

ABSTRACT: The protozoan parasite Trypanosoma cruzi causes Chagas disease, one of the major neglected infectious diseases. It has the potential to infect any nucleated mammalian cell. The secreted/excreted protein repertoire released by T. cruzi trypomastigotes is crucial in host-pathogen interactions. In this study, mammalian tissue culture-derived trypomastigotes (Y strain) were used to characterize the exoproteome of the infective bloodstream life form. Proteins released into the serum-free culture medium after 3 h of incubation were harvested and digested with trypsin. NanoLC-MS/MS analysis resulted in the identification of 540 proteins, the largest set of released proteins identified to date in Trypanosoma spp. Bioinformatic analysis predicted most identified proteins as sec

SUBMITTER: Queiroz RM 

PROVIDER: S-EPMC5097913 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature