MTOR Overactivation in Mesenchymal cells Aggravates CCl₄- Induced liver Fibrosis.

ABSTRACT: Hepatic stellate cells are of mesenchymal cell type located in the space of Disse. Upon liver injury, HSCs transactivate into myofibroblasts with increase in expression of fibrillar collagen, especially collagen I and III, leading to liver fibrosis. Previous studies have shown mTOR signaling is activated during liver fibrosis. However, there is no direct evidence in vivo. The aim of this study is to examine the effects of conditional deletion of TSC1 in mesenchymal on pathogenesis of liver fibrosis. Crossing mice bearing the floxed TSC1 gene with mice harboring Col1α2-Cre-ER(T) successfully generated progeny with a conditional knockout of TSC1 (TSC1 CKO) in collagen I expressing mesenchymal cells. TSC1 CKO and WT mice were subjected to CCl₄, oil or CCl₄+ rapamycin tre

SUBMITTER: Shan L 

PROVIDER: S-EPMC5098141 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature