The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells.

ABSTRACT: Some mutant forms of the cellular prion protein (PrP^C) carrying artificial deletions or point mutations associated with familial human prion diseases are capable of inducing spontaneous ionic currents across the cell membrane, conferring hypersensitivity to certain antibiotics to a wide range of cultured cells and primary cerebellar granular neurons (CGNs). These effects are abrogated when the wild type (WT) form is co-expressed, suggesting that they might be related to a physiological activity of PrP^C. Interestingly, the prion protein family member Shadoo (Sho) makes cells hypersensitive to the same antibiotics as mutant PrP-s, an effect that is diminished by the co-expression of WT-PrP. Here, we report that Sho engages in another mutant PrP-like activity: it spontan

SUBMITTER: Nyeste A 

PROVIDER: S-EPMC5098206 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature