Distribution and Quantification of Choroidal Macrophages in Human Eyes With Age-Related Macular Degeneration.

ABSTRACT:

Purpose

Increasing evidence suggests a role for macrophages in the pathogenesis of age-related macular degeneration (AMD). This study examined choroidal macrophages and their activation in postmortem eyes from subjects with and without AMD.

Methods

Choroids were incubated with anti-ionized calcium-binding adapter molecule 1 (anti-IBA1) to label macrophages, anti-human leukocyte antigen-antigen D-related (anti-HLA-DR) as a macrophage activation marker, and Ulex europaeus agglutinin lectin to label blood vessels. Whole mounts were imaged using confocal microscopy. IBA1- and HLA-DR-positive (activated) cells were counted in submacula, paramacula, and nonmacula, and cell volume and sphericity were determined using computer-assisted image analysis.

Results

In aged control

SUBMITTER: McLeod DS 

PROVIDER: S-EPMC5098452 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature