Cytosolic phospholipase A₂α increases proliferation and de-differentiation of human renal tubular epithelial cells.

ABSTRACT: The group IVA calcium-dependent cytosolic phospholipase A₂ (cPLA₂α) enzyme controls the release of arachidonic acid from membrane bound phospholipids and is the rate-limiting step in production of eicosanoids. A variety of different kidney injuries activate cPLA₂α, therefore we hypothesized that cPLA₂α activity would regulate pathologic processes in HK-2 cells, a human renal tubular epithelial cell line, by regulating cell phenotype and proliferation. In two lentiviral cPLA₂α-silenced knockdowns, we observed decreased proliferation and increased apoptosis compared to control HK-2 cells. cPLA₂α-silenced cells also demonstrated an altered morphology, had increased expression E-cadherin, and decreased expression of Ncadherin. I

SUBMITTER: Montford JR 

PROVIDER: S-EPMC5099119 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature