Imaging the NADH:NAD⁺ Homeostasis for Understanding the Metabolic Response of Mycobacterium to Physiologically Relevant Stresses.

ABSTRACT: The NADH:NAD⁺ ratio is the primary indicator of the metabolic state of bacteria. NAD(H) homeostasis is critical for Mycobacterium tuberculosis (Mtb) survival and is thus considered an important drug target, but the spatio-temporal measurements of NAD(H) remain a challenge. Genetically encoded fluorescent biosensors of the NADH:NAD⁺ ratios were recently described, paving the way for investigations of the metabolic state of pathogens during infection. Here we have adapted the genetically encoded biosensor Peredox for measurement of the metabolic state of Mtb in vitro and during infection of macrophage cells. Using Peredox, here we show that inhibition of the electron transport chain, disruption of the membrane potential and proton gradient, exposure to rea

SUBMITTER: Bhat SA 

PROVIDER: S-EPMC5099167 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature