An RNA-binding protein, RNP-1, protects microtubules from nocodazole and localizes to the leading edge during cytokinesis and cell migration in Dictyostelium cells.

ABSTRACT:

Aim

RNA-binding proteins are a large group of regulators (800-1000 in humans), some of which play significant roles in mRNA local translation. In this study, we analyzed the functions of the protein RNP-1, which was previously discovered in a genetic selection screen for nocodazole suppression.

Methods

The growth rates and the microtubule networks of Dictyostelium cells were assessed with or without nocodazole (10 μmol/L) in suspension culture. Fluorescent images of RNP-1-GFP and RFP-tubulin were captured when cells were undergoing cytokinesis, then the GFP signal intensity and distance to the nearest centrosome were analyzed by using a computer program written in Matlab^®. The RNP-1-GFP-expresseding cells were polarized, and the time-lapse images of cells were cap

SUBMITTER: Ngo T 

PROVIDER: S-EPMC5099410 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature