Chlorogenic acid analogues from Gynura nepalensis protect H9c2 cardiomyoblasts against H₂O₂-induced apoptosis.

ABSTRACT:

Aim

Chlorogenic acid has shown protective effect on cardiomyocytes against oxidative stress-induced damage. Herein, we evaluated nine caffeoylquinic acid analogues (1-9) isolated from the leaves of Gynura nepalensis for their protective effect against H₂O₂-induced H9c2 cardiomyoblast damage and explored the underlying mechanisms.

Methods

H9c2 cardiomyoblasts were exposed to H₂O₂ (0.3 mmol/L) for 3 h, and cell viability was detected with MTT assay. Hoechst 33342 staining was performed to evaluate cell apoptosis. MMPs (mitochondrial membrane potentials) were measured using a JC-1 assay kit, and ROS (reactive oxygen species) generation was measured using CM-H₂ DCFDA. The expression levels of relevant proteins were detected

SUBMITTER: Yu BW 

PROVIDER: S-EPMC5099414 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature