Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1.

ABSTRACT: The tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMP_TP901-1) was undertaken. We generated 56 mutants aimed at defining TMP_TP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMP_TP901-1 as a minimum. Furthermore, the role of TMP_TP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mu

SUBMITTER: Mahony J 

PROVIDER: S-EPMC5099701 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature