Peripheral and spinal 5-HT receptors participate in cholestatic itch and antinociception induced by bile duct ligation in rats.

ABSTRACT: Although 5-HT has been implicated in cholestatic itch and antinociception, two common phenomena in patients with cholestatic disease, the roles of 5-HT receptor subtypes are unclear. Herein, we investigated the roles of 5-HT receptors in itch and antinociception associated with cholestasis, which was induced by common bile duct ligation (BDL) in rats. 5-HT-induced enhanced scratching and antinociception to mechanical and heat stimuli were demonstrated in BDL rats. 5-HT level in the skin and spinal cord was significantly increased in BDL rats. Quantitative RT-PCR analysis showed 5-HT_1B, 5-HT_1D, 5-HT_2A, 5-HT_3A, 5-HT_5B, 5-HT₆, and 5-HT₇ were up-regulated in peripheral nervous system and 5-HT_1A, 5-HT_1F

SUBMITTER: Tian B 

PROVIDER: S-EPMC5099756 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature