Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana.

ABSTRACT: Live imaging of the dynamics of nuclear organization provides the opportunity to uncover the mechanisms responsible for four-dimensional genome architecture. Here, we describe the use of fluorescent protein (FP) fusions of transcription activator-like effectors (TALEs) to visualize endogenous genomic sequences in Arabidopsis thaliana. The ability to engineer sequence-specific TALEs permits the investigation of precise genomic sequences. We could detect TALE-FP signals associated with centromeric, telomeric, and rDNA repeats and the signal distribution was consistent with that observed by fluorescent in situ hybridization. TALE-FPs are advantageous because they permit the observation of intact tissues. We used our TALE-FP method to investigate the nuclei of several multicellular plant tissu

SUBMITTER: Fujimoto S 

PROVIDER: S-EPMC5100022 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature