Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs.

ABSTRACT: Acidic mammalian chitinase (AMCase) is implicated in asthma, allergic inflammation, and food processing. Little is known about genetic and evolutional regulation of chitinolytic activity of AMCase. Here, we relate human AMCase polymorphisms to the mouse AMCase, and show that the highly active variants encoded by nonsynonymous single-nucleotide polymorphisms (nsSNPs) are consistent with the mouse AMCase sequence. The chitinolytic activity of the recombinant human AMCase was significantly lower than that of the mouse counterpart. By creating mouse-human chimeric AMCase protein we found that the presence of the N-terminal region of human AMCase containing conserved active site residues reduced the enzymatic activity of the molecule. We were able to significantly increase the activity of human

SUBMITTER: Okawa K 

PROVIDER: S-EPMC5100053 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature