Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections.

ABSTRACT:

Background

Necrotizing soft tissue infections (NSTIs) are a group of infections affecting all soft tissues. NSTI involves necrosis of the afflicted tissue and is potentially life threatening due to major and rapid destruction of tissue, which often leads to septic shock and organ failure. The gold standard for identification of pathogens is culture; however molecular methods for identification of microorganisms may provide a more rapid result and may be able to identify additional microorganisms that are not detected by culture.

Methods

In this study, tissue samples (n = 20) obtained after debridement of 10 patients with NSTI were analyzed by standard culture, fluorescence in situ hybridization (FISH) and multiple molecular methods. The molecular methods included analysis of

SUBMITTER: Rudkjobing VB 

PROVIDER: S-EPMC5100109 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature