Intracellular TRPA1 mediates Ca2+ release from lysosomes in dorsal root ganglion neurons.

ABSTRACT: Transient receptor potential A1 (TRPA1) is a nonselective cation channel implicated in thermosensation and inflammatory pain. In this study, we show that TRPA1 (activated by allyl isothiocyanate, acrolein, and 4-hydroxynonenal) elevates the intracellular Ca²⁺ concentration ([Ca²⁺]_i) in dorsal root ganglion (DRG) neurons in the presence and absence of extracellular Ca²⁺ Pharmacological and immunocytochemical analyses revealed the presence of TRPA1 channels both on the plasma membrane and in endolysosomes. Confocal line-scan imaging demonstrated Ca²⁺ signals elicited from individual endolysosomes ("lysosome Ca²⁺ sparks") by TRPA1 activation. In physiological solutions, the TRPA1-mediated endolysosomal Ca²⁺ release c

SUBMITTER: Shang S 

PROVIDER: S-EPMC5100290 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature