Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.

ABSTRACT: During cardiovascular development, tight spatiotemporal regulation of molecular cues is essential for controlling endothelial cell (EC) migration. Secreted class III Semaphorins play an important role in guidance of neuronal cell migration and were lately linked to regulating cardiovascular development. Recently, SEMA3D gene disruptions were associated with cardiovascular defects in patients; however, the mechanisms of action were not revealed. Here we show for the first time that Sema3d regulates collective EC migration in zebrafish through two separate mechanisms. Mesenchymal Sema3d guides outgrowth of the common cardinal vein via repulsion and signals through PlexinD1. Additionally, within the same ECs, we identified a novel function of autocrine Sema3d signaling in regulating Actin net

SUBMITTER: Hamm MJ 

PROVIDER: S-EPMC5100291 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature