Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases.

ABSTRACT: RNA-protein complexes play a central role in the regulation of fundamental cellular processes, such as mRNA splicing, localization, translation and degradation. The misregulation of these interactions can cause a variety of human diseases, including cancer and neurodegenerative disorders. Recently, many strategies have been developed to comprehensively analyze these complex and highly dynamic RNA-protein networks. Extensive efforts have been made to purify in vivo-assembled RNA-protein complexes. In this review, we focused on commonly used RNA-centric approaches that involve mass spectrometry, which are powerful tools for identifying proteins bound to a given RNA. We present various RNA capture strategies that primarily depend on whether the RNA of interest is modified. Moreover, we briefl

SUBMITTER: Jazurek M 

PROVIDER: S-EPMC5100574 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature