Synthesis and in Vitro and in Vivo Evaluation of MMP-12 Selective Optical Probes.

ABSTRACT: In designing new tracers consisting of a small peptide conjugated to a reporter of comparable size, particular attention needs to be paid to the selection of the reporter group, which can dictate both the in vitro and the in vivo performances of the whole conjugate. In the case of fluorescent tracers, this is particularly true given the large numbers of available dye moieties differing in their structures and properties. Here, we have investigated the in vitro and in vivo properties of a novel series of MMP-12 selective probes composed of cyanine dyes varying in their structure, net charge, and hydrophilic character, tethered through a linker to a potent and specific MMP-12 phosphinic pseudopeptide inhibitor. The impact of linker length has been also explored. The crystallographic structur

SUBMITTER: Bordenave T 

PROVIDER: S-EPMC5100701 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature