Temporal Expression of a Master Regulator Drives Synchronous Sporulation in Budding Yeast.

ABSTRACT: Yeast cells enter and undergo gametogenesis relatively asynchronously, making it technically challenging to perform stage-specific genomic and biochemical analyses. Cell-to-cell variation in the expression of the master regulator of entry into sporulation, IME1, has been implicated to be the underlying cause of asynchronous sporulation. Here, we find that timing of IME1 expression is of critical importance for inducing cells to undergo sporulation synchronously. When we force expression of IME1 from an inducible promoter in cells incubated in sporulation medium for 2 hr, the vast majority of cells exhibit synchrony during premeiotic DNA replication and meiotic divisions. Inducing IME1 expression too early or too late affects the synchrony of sporulation. Surpris

SUBMITTER: Chia M 

PROVIDER: S-EPMC5100854 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature