Initial Characterization of the Pig Skin Bacteriome and Its Effect on In Vitro Models of Wound Healing.

ABSTRACT: Elucidating the roles and composition of the human skin microbiome has revealed a delicate interplay between resident microbes and wound healing. Evolutionarily speaking, normal cutaneous flora likely has been selected for because it potentiates or, at minimum, does not impede wound healing. While pigs are the gold standard model for wound healing studies, the porcine skin microbiome has not been studied in detail. Herein, we performed 16S rDNA sequencing to characterize the pig skin bacteriome at several anatomical locations. Additionally, we used bacterial conditioned-media with in vitro techniques to examine the paracrine effects of bacterial-derived proteins on human keratinocytes (NHEK) and fibroblasts (NHDF). We found that at the phyla level, the pig skin bacteriome is similar to tha

SUBMITTER: McIntyre MK 

PROVIDER: S-EPMC5100914 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature