Mass spectrometric phosphoproteome analysis of HIV-infected brain reveals novel phosphorylation sites and differential phosphorylation patterns.

ABSTRACT:

Purpose

To map the phosphoproteome and identify changes in the phosphorylation patterns in the HIV-infected and uninfected brain.

Experimental design

Parietal cortex from individuals with and without HIV infection were lysed and trypsinized. The peptides were labeled with iTRAQ reagents, combined, phospho-enriched by titanium dioxide chromatography, and analyzed by LC-MS/MS with high resolution.

Results

Our phosphoproteomic workflow resulted in the identification of 112 phosphorylated proteins and 17 novel phosphorylation sites in all the samples that were analyzed. The phosphopeptide sequences were searched for kinase substrate motifs, which revealed potential kinases involved in important signaling pathways. The site-specific phosphopeptide quantification showed tha

SUBMITTER: Uzasci L 

PROVIDER: S-EPMC5101124 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature