Restoration of Hydrogen Sulfide Production in Diabetic Mice Improves Reparative Function of Bone Marrow Cells.

ABSTRACT:

Background

Bone marrow cell (BMC)-based treatment for critical limb ischemia in diabetic patients yielded a modest therapeutic effect resulting from cell dysfunction. Therefore, approaches that improve diabetic stem/progenitor cell functions may provide therapeutic benefits. Here, we tested the hypothesis that restoration of hydrogen sulfide (H₂S) production in diabetic BMCs improves their reparative capacities.

Methods

Mouse BMCs were isolated by density-gradient centrifugation. Unilateral hind limb ischemia was conducted in 12- to 14-week-old db/+ and db/db mice by ligation of the left femoral artery. The H₂S level was measured by either gas chromatography or staining with florescent dye sulfidefluor 7 AM.

Results

Both H₂S production

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC5101140 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature