Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.

ABSTRACT: Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. ChIP-SICAP in mouse embryonic stem cells (ESCs) identified over 400 proteins associating with OSN, including several whose interaction depends on the pluripotent state. Trim24, a previously unrecognized protein in the network, converges with OSN on multiple enhancers and suppresses the expression of developmental genes while activating

SUBMITTER: Rafiee MR 

PROVIDER: S-EPMC5101186 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature