Vibrational stimulation induces osteoblast differentiation and the upregulation of osteogenic gene expression in vitro.

ABSTRACT: Vibrational stimulation is an accepted non-invasive method used to improve bone remodeling. However, the underlying mechanisms of this phenomenon remain unclear. In this study, we developed a new vibration-loading system to apply vibrational stimulation to cells based on a previously reported in vivo study. We hypothesized that osteoblasts respond to vibrational strain by expressing osteogenic marker genes, such as alkaline-phosphatase (ALP), Runx2, and Osterix. To test our hypothesis, we developed a vibration-loading system to apply a precise vibrational force to an osteoblast culture on a silicone membrane. The system regulated frequency and acceleration of the vibration, and strain on the silicone membrane culture surface was measured using the strain gauge method. After vibrational sti

SUBMITTER: Ota T 

PROVIDER: S-EPMC5101299 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature