One Gene and Two Proteins: a Leaderless mRNA Supports the Translation of a Shorter Form of the Shigella VirF Regulator.

ABSTRACT: VirF, an AraC-like activator, is required to trigger a regulatory cascade that initiates the invasive program of Shigella spp., the etiological agents of bacillary dysentery in humans. VirF expression is activated upon entry into the host and depends on many environmental signals. Here, we show that the virF mRNA is translated into two proteins, the major form, VirF₃₀ (30 kDa), and the shorter VirF₂₁ (21 kDa), lacking the N-terminal segment. By site-specific mutagenesis and toeprint analysis, we identified the translation start sites of VirF₃₀ and VirF₂₁ and showed that the two different forms of VirF arise from differential translation. Interestingly, in vitro and in vivo translation experiments showed that VirF₂₁ is also translated f

SUBMITTER: Di Martino ML 

PROVIDER: S-EPMC5101355 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature